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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  DNA_Clean-Up >  PCR_Clean-Up >  Silica_Beads

PCR Clean-Up Silica Resin-Based Kits—NucleoTraPCR PCR Purification Kit

NucleoTraPCR Kits allow you to extract DNA fragments from PCR reactions. Each kit yields exceptionally pure DNA fragments for use in ligations, PCR, cloning, and probe construction. The entire NucleoTrap purification procedure takes only 45 minutes.

NucleoTrap resin is a spherical silica matrix in aqueous suspension. Because of the uniformity of the silica particles, even large DNA fragments (<20 kb) are safe from shearing and degradation.

The NucleoTraPCR PCR purification kit contains a modified matrix that cannot bind fragments smaller than 100 bp. It is ideal for separating PCR products from primers, primer-dimers, and free nucleotides after PCR is completed. Each 10 µl of suspension can extract 6 µg of DNA.

At-A-Glance Documents Images & Data Resources

Features

  • Quantitative removal of primer and primer-dimer molecules
  • High recovery for DNA fragments >100 bp

Applications

Purified DNA is suitable for:

  • Cloning
  • Sequencing
  • PCR
  • Restriction analysis

Product Overview

Technology Silica beads
Format Silica bead suspension
Starting Material 20–400 µl PCR reaction mixture
Fragment Size 100 bp–20 kb
Typical Recovery 70–80%
A260/280 1.7–1.9
Elution Volume 20–50 µl
Preparation Time 45 min/6 preps
Binding Capacity 6 µg/10 µl suspension

 
 
Products
Cat. # Product Package Size Price # of Units Select
740587 NucleoTraP®CR 100 Preps $95.00
 

 

 


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