The NucleoFast 96 PCR Clean-Up Kit and NucleoFast 96 PCR Plates use ultrafiltration for high recovery of nucleic acids in less than 20 minutes. The nucleic acids from the PCR reaction are collected on the filter membrane while the contaminants are filtered directly to the waste. The NucleoFast protocol is faster because it eliminates the intermediate wash steps required with silica-based purification systems. The 96 PCR Clean-Up Kit includes buffers and elution plate. The sturdy, one-piece plate is easily adapted to automated processing. The purified PCR products are suitable for most common downstream applications, including capillary sequencing, microarray spotting, restriction analysis, labeling, or cloning.
Magnetic PCR Clean-up Kits—96-Well Plates
NucleoMag 96 PCR Kits are magnetic bead-based kits for parallel purification of PCR products, which provide a reliable, cost- and labor-efficient tool. PCR products are selectively bound to the NucleoMag PCR Beads, while contaminants (e.g., primers, dNTPs, enzymes, salts) are washed away. Purified PCR products are eluted from the beads under low-salt conditions and are ready to use in downstream applications such as sequencing or microarray spotting. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), there is no risk of cross-contamination. The NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.