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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  DNA_Clean-Up >  PCR_Clean-Up >  Enzyme_Removal

Resin-Based Enzyme Removal from DNA & RNA Solutions—QuickClean Enzyme Removal Resin

QuickClean Enzyme Removal Resin is a solid-phase matrix for phenol-free removal of proteins from aqueous solutions of single- or double-stranded nucleic acids used for quick, phenol-free deproteinization of DNA and RNA samples. QuickClean efficiently removes enzymes and other proteins from DNA or RNA samples in a brief vortex and spin step. No hazardous phenol extractions or time-consuming ethanol precipitations are required. The simple protocol can be performed in less than five minutes, and the resin’s blue color makes it easier to remove.

QuickClean Enzyme Removal Resin may be used to remove restriction enzymes, ligases, Taq DNA polymerase, RNase, and DNase after reactions with DNA or RNA to prevent those enzymes from interfering with subsequent manipulations. The resin can also be used to deproteinize DNA and RNA before electrophoresis on agarose gels. This will improve the resolution and clarity of bands when preparing for blot hybridization. If bands are to be cut out of the gel, deproteinization before electrophoresis will eliminate enzymes that may comigrate with the DNA fragments and coelute from the gel slices.

At-A-Glance Documents Images & Data Resources

Features

  • Phenol-free protein removal from aqueous DNA & RNA solutions
  • Rapid protocol takes less than 5 min
  • Blue color of resin makes it easier to remove

Applications

  • Removal of enzymes from DNA and RNA reaction mixtures after completion, to prevent interference with downstream applications
  • Deproteinization of DNA and RNA before agarose gel electrophoresis in order to:
    • Improve band resolution and clarity when preparing for blot hybridization
    • Eliminate comigrating proteins that coelute with gel slices prior to gel extraction

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
631770 QuickClean Enzyme Removal Resin 0.5 mL $187.00
 

 

 


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