The NucleoSpin 96 PCR Clean-Up Kits combine an improved buffer chemistry with the design of the 96-well plates and allow the direct purification of PCR products using vacuum manifolds or benchtop centrifuges. The kits allow the rapid clean-up of up to 15 µg of DNA (65 bp–10 kb) per preparation with high DNA recovery and reproducible yields. After purification, the highly pure PCR products are ready for immediate use in many applications.
NucleoSpin PCR Clean-Up Binding Plates can be used under vacuum or by centrifugation. The NucleoSpin 96 PCR Clean-Up Kit uses advanced silica-membrane technology. The addition of a binding buffer containing chaotropic ions leads to a reversible adsorption of DNA to the silica membrane. PCR products bind to the silica membrane under optimized conditions, while contaminants like primers, salts, nucleotides, and proteins are completely removed by effective washing. Highly pure PCR products are eluted with low-salt elution buffer (5 mM Tris/HCl pH 8.5) or water (pH 8.0–8.5) and are ready-to-use directly for subsequent applications.