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Products >  Nucleic_Acid_Purification >  DNA_Clean-Up >  PCR_Clean-Up >  96-Well_Plate

PCR Clean-Up 96-Well Plates

The NucleoSpin 96 PCR Clean-Up Kits combine an improved buffer chemistry with the design of the 96-well plates and allow the direct purification of PCR products using vacuum manifolds or benchtop centrifuges. The kits allow the rapid clean-up of up to 15 µg of DNA (65 bp–10 kb) per preparation with high DNA recovery and reproducible yields. After purification, the highly pure PCR products are ready for immediate use in many applications.

NucleoSpin PCR Clean-Up Binding Plates can be used under vacuum or by centrifugation. The NucleoSpin 96 PCR Clean-Up Kit uses advanced silica-membrane technology. The addition of a binding buffer containing chaotropic ions leads to a reversible adsorption of DNA to the silica membrane. PCR products bind to the silica membrane under optimized conditions, while contaminants like primers, salts, nucleotides, and proteins are completely removed by effective washing. Highly pure PCR products are eluted with low-salt elution buffer (5 mM Tris/HCl pH 8.5) or water (pH 8.0–8.5) and are ready-to-use directly for subsequent applications.

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Features

  • Time-saving parallel clean-up of PCR products
  • Complete removal of primers and primer-dimers
  • Optimal recovery
  • Suitable for processing under vacuum and centrifugation
  • Innovative MN Wash Plate minimizes risk of cross-contamination
  • Suitable for manual or automated use

Applications

Radioactive and fluorescent dye sequencing, including capillary sequencing, restriction analysis, ligation, and microarray analysis.

Product Overview

TechnologySilica membrane
Format96-well plates
ProcessingManual or automated,
vacuum or centrifugation
Starting Material<100 µl PCR reaction mixture
Fragment Size65 bp–10 kb
Typical Recovery75–95%
A260/2801.70–1.80
Elution Volume75–150 µl
Preparation Time45 min/plate
Binding Capacity15 µg

 
 
Products
Cat. # Product Contents Size Price Units Select
740658.1 null 1 x 96 Preps $256.00
740658.2 null 2 x 96 Preps $420.00
740658.4 null 4 x 96 Preps $633.00
740658.24 null 24 x 96 Preps $2,558.00
740658.24S null 24 Plates $1,535.00
740464.4 null 4 x 96 Preps $615.00
 

 

 
 

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