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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  DNA_Clean-Up >  Genomic_DNA_Clean-Up

Clean-Up and Concentration of Large Genomic DNA Fragments

The NucleoSpin gDNA Clean-Up and NucleoSpin gDNA Clean-Up XS kits provide the easiest and fastest way to clean up and concentrate large genomic DNA fragments from enzymatic reactions or prepurified samples (extracted using organic solvents such as phenol/chloroform)—or to perform buffer exchange. These kits can be used to remove contaminants such as salts, enzymes, nucleotides, labeling reagents, etc. by selective binding to a silica membrane in a spin column format, using a simple bind-wash-elute procedure. The proven silica membrane technology is an effective alternative to the widely used Centricon concentrator columns for cleaning and concentrating samples extracted by standard “organic” methods.

NucleoSpin gDNA Clean-Up includes mini spin columns (50–100 µl elution volume) to purify standard samples (< 150 µl aqueous DNA solution containing < 25 µg DNA), while NucleoSpin gDNA Clean-Up XS includes XS columns (6–10 µl elution volume) to purify very small samples (< 400 µl aqueous DNA solution containing < 2 µg DNA). The NucleoSpin gDNA Clean-Up XS Column contains a unique silica membrane that allows the smallest elution volumes. Due to its high sensitivity, the XS kit is particularly well-suited for purifying trace levels of DNA from forensic samples. These kits reduce sample preparation time to 15–20 min, compared to > 1 hr for ultrafiltration devices. The purified DNA is suitable for a variety of downstream applications, including PCR, enzymatic reactions, Southern blotting, qPCR, and STR amplification.

At-A-Glance Documents Images & Data

Features

  • Efficient, effective clean-up and concentration of large DNA fragments for downstream applications
  • Easier and faster sample concentration than microdialysis filtration units
  • Two kit options—mini columns for standard samples and XS columns for very small (e.g., forensic) samples
  • Get high recovery rates (up to 90%) with NucleoSpin gDNA Clean-Up
  • Get highly concentrated DNA (elute in 6–10 µl) with NucleoSpin gDNA Clean-Up XS

Applications

  • Clean-up and concentration of prepurified DNA (organic extractions)
  • Clean-up and concentration of DNA from enzymatic reactions*
  • Typical downstream applications include PCR, enzymatic reactions, Southern blotting, qPCR, STR amplification

* Not recommended for gel or PCR clean-up! Use NucleoSpin Gel and PCR Clean-up for these applications.

Product Overview

NucleoSpin gDNA Clean-Up

NucleoSpin gDNA Clean-Up XS

Technology

Silica membrane

Silica membrane

Format

Mini spin columns

Mini spin columns–XS design

Starting Material

<150 µl DNA solution
(<25 µg DNA)

<400 µl DNA solution
(<2 µg DNA)

Fragment Size

100 bp–approx. 50 kb

100 bp–approx. 50 kb

Typical Recovery

80–90%

60–70%

A260/A280

1.8–1.9

1.8–1.9

Elution Volume

50–100 µl

6–10 µl

Preparation Time

15 min/10 preps

20 min/6 preps

Binding Capacity

50 µg

3 µg


 
 
Products
Offers Cat. # Product Package Size Price # of Units Select
  740230.10 NucleoSpin® gDNA Clean-up 10 Preps $30.00
740230.50 NucleoSpin® gDNA Clean-up 50 Preps $104.00
740230.250 NucleoSpin® gDNA Clean-up 250 Preps $482.00
  740904.10 NucleoSpin® gDNA Clean-up XS 10 Preps $30.00
740904.50 NucleoSpin® gDNA Clean-up XS 50 Preps $120.00
740904.250 NucleoSpin® gDNA Clean-up XS 250 Preps $566.00
 

 

 

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