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Nucleic Acid Purification


Products >  Nucleic_Acid_Purification >  DNA_Clean-Up >  Gel_Extraction >  Spin

Gel Extraction and PCR Clean-Up Kit

With the NucleoSpin Gel Extraction and PCR Clean-Up Kit you get two applications in one. The supplied column, which is suitable for gel extraction as well as PCR purification, binds DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded directly onto the NucleoSpin Gel and PCR Clean-Up Kit column, only a single wash step using Buffer NT3, an optimized buffer, is necessary—providing rapid DNA purification. You can isolate fragments from 65 bp–10 kb in a minimized elution volume (as low as 15 µl) with a recovery of 70–95%. Downstream applications include sequencing, ligation, labeling, cloning, and restriction enzyme digestion.

The NucleoSpin Gel and PCR Clean-Up Kit was formerly known as NucleoSpin Extract II Kit.

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Features

  • ONE kit for PCR clean-up and gel extraction
  • Highly flexible—kit can be used for:
    • Small to large fragments (65 bp to 10 kb)
    • Low and standard elution volumes (elute in as little as 15 µl)
    • Restriction digest clean-up
    • Samples containing detergent (kit has no detergent sensitivity)
  • Fast—no isopropanol precipitation step for PCR purification
  • Effective removal of primers

Applications

Purified DNA is suitable for:

  • Sequencing
  • Ligation
  • Labeling
  • Cloning
  • Restriction enzyme digestion

Product Overview

Technology Silica membrane
Format Mini spin columns
Starting Material <150 µl PCR reaction mixture
<400 mg TAE/TBE agarose gel
Fragment Size 65 bp–10 kb
Typical Recovery 70–95%
A260/280 1.8–1.9
Elution Volume 15–50 µl
Preparation Time 10 min/6 preps
Binding Capacity 25 µg

 
 
Products
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Offers Cat. # Product Contents Size Price Units Select
740609.250 NucleoSpin® Gel and PCR Clean-Up 250 Preps $365.00
740609.50 NucleoSpin® Gel and PCR Clean-Up
      
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