CHROMA SPIN Columns are spin columns packed with gel filtration resin to rapidly purify and size select nucleic acid samples. The columns can be used to purify single- or double-stranded DNA or RNA from contaminants such as salts, solvents, enzymes, or proteins. Molecules larger than the particular matrix pore size are eluted out of the column while smaller molecules are retained inside the column matrix. As a result, these columns are ideal for size fractionation of libraries or for removal of primers.
Available in Three Different Sterile Buffers
- TE buffer: ideal for routine DNA applications
- STE (0.1 M NaCl + TE) buffer: used for applications requiring a higher salt concentration to prevent ionic interaction between molecules
- DEPC-treated water (+ 0.1 mM EDTA): useful for RNA purification and those applications requiring a completely nuclease-free environment
Choose from Six Column Pore Sizes
CHROMA SPIN-10, -30, -100, -200, -400, and -1000. All columns, matrices, and buffers have been autoclaved to destroy nucleases. For optimal recovery and purification, there should be at least a threefold difference between the size of the desired nucleic acid molecules and the size of the contaminants.