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Molecular Biology Tools

Products >  Molecular_Biology_Tools >  Restriction_Enzymes >  Restriction_Enzymes_mn >  NsbI

NsbI (MstI, AviII, FspI)

  Restriction enzyme NsbI (NsbI) | Isoschizomer of Acc16I, AviII, FspI, MstI, AviII DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.


DNA Restriction Enzyme: NsbI (MstI, AviII, FspI)

  • Source: Neisseria subtlava Va-1
  • Concentration: 4-12 units/µl
  • Supplied buffer: T+BSA
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Effect of DNA methylation: Enzyme activity is affected by CG methylase. 
  • Star activity: Cleavage at an alternative site occurs in the presence of Mn2+.
  • Genomic DNA analysis: Anthrobacter luteus chromosomal DNA

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.

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