DNA Restriction Enzyme: EcoRV
- Source: Escherichia coli
- Concentration: 8-20 units/µl (high concentration: 30-50 units/µl)
- Supplied buffer: H
- Reaction temperature: 37°C Substrate for unit definition: lambda DNA
- Effect of DNA methylation: Enzyme activity is not affected by CG methylase.
- Star activity: Unrelated site may often be cut in the presence of DMSO.
TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning