DNA Restriction Enzyme: Eco81I (SauI)
- Source: Escherichia coli RFL 8
- Supplied buffer: M
- Reaction temperature: 37°C
- Substrate for unit definition: lambda DNA
- Ligation-recutting test: After digestion with this enzyme, ligation is inefficient due to the structure of the Eco81 I fragment ends (Janulaitis, A.A., personal communication). For circularized ligation, the overnight ligation using TaKaRa DNA Ligation Kit, Ver.1 or Ver.2.1 or Mighty Mix is recommended.
TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.