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Molecular Biology Tools

Products >  Molecular_Biology_Tools >  Restriction_Enzymes >  Restriction_Enzymes_e_f >  Eam1105I


DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

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DNA Restriction Enzyme: Eam1105I

  • Source: Enterobacter amnigenes RFL 1105
  • Concentration: 2-10 units/µl
  • Supplied buffer: Basal
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Ligation-recutting test: Ligation efficiency of cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved using blunt end ligation reaction conditions.
  • Effect of DNA methylation: Enzyme activity is not affected by CG methylase.
  • Star activity: Unrelated site may often be cut in the presence of high concentration of glycerol, and at alkaline pH or low ionic strength.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.

Cat. # Product Contents Size Price Units Select
1124A Eam1 105 I 100 Units $85.00
1124B Eam1 105 I 500 Units $335.00


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