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Products >  Molecular_Biology_Tools >  Restriction_Enzymes >  Restriction_Enzymes_c_d >  DdeI

DdeI

Takara DNA restriction enzymes  such as Dde I are unsurpassed in quality and purity. We’ve been producing restriction enzymes for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara-brand DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit the Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

  At-A-Glance   Documents

Features

DNA Restriction Enzyme: DdeI
C|TNA G
G ANT|C
  • Source: E. coli carrying a plasmid encoding the DdeI gene
  • Supplied buffer: K
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Star activity: Digestion at unrelated site(s) may often occur in the presence of high concentration of glycerol, Mn2+, or DMSO.
  • Inactivation: fully inactivated by incubation at 70°C for 15 min. Alternatively, may be inactivated by ethanol precipitation, phenol treatment, or by adding 1/10 volume of 10X Loading Buffer (supplied) prior to loading directly on an agarose gel for analysis.

Composition of 10X Loading Buffer

0.9% SDS
50% glycerol
0.05% Bromophenyl Blue

Store 10X Loading Buffer at room temperature. SDS may precipitate during storage. If precipitates are present, dissolve by incubating briefly at 37° before use.

Quality control

Takara follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.


 
 
Products
Cat. # Product Contents Size Price Units Select
1248A Dde l 500 Units $85.00
1248B Dde l 2,500 Units $424.00
 

 

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