DNA Restriction Enzyme: Bpu1102 I (Esp I)
- Source: Bacillus pumilus
- Concentration: 10 units/ul
- Supplied buffer: Basal
- Reaction temperature: 37°C
- Substrate for unit definition: lambda DNA
- Ligation-recutting test: Ligation efficiency of DNA fragments with cohesive end generated by this enzyme is lower than general 3-base protruding ends. Therefore, more efficient ligation can be achieved by using the reaction conditions for blunt end ligation.
TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning