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BciT130 (EcoRII, MvaI)

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

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DNA Restriction Enzyme: BciT130 (EcoRII, MvaI)

  • Source: Bacillus circulans T130
  • Concentration: 10 units/ul
  • Supplied buffer: K
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Inactivation treatment: Heat treatment (70°C, 15 min) is not sufficient to inactivate this enzyme completely. It is necessary to inactivate this enzyme by ethanol precipitation treatment (or phenol treatment--ethanol precipitation treatment).
  • Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of Bci T130 I fragment ends. For ligation, the overnight ligation using DNA Ligation Kit Ver.2.1 is recommended.
  • Effect of DNA methylation: Enzyme activity is not affected by dcm methylase.
  • Star Activity: Unrelated site may be cut in the presence of high concentrations of glycerol, DMSO, or low ionic strength.

    TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning

    Cat. # Product Contents Size Price Units Select
    1232A BciT130 I (EcoR II, Mva I) 2,000 Units $122.00
    1232B BciT130 I (EcoR II, Mva I) 10,000 Units $488.00


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