Restriction Enzyme AccIII (Acc III, BspMII) | Isoschizomer of Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

Features

DNA Restriction Enzyme: AccIII (BspMII)

CCGG

GGCC

Source: Acinetobacter calcoaceticus

Concentration: 1-5 units/ul

Supplied Buffer: Basal

Effect of DNA Methylation: This enzyme is capable of cleaving TC^5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase depending on the sequence following the recognition site.

Reaction Temperature: 60°C

Substrate for Unit Definition: lambda DNA

Cleavage of pBR322: Commercially available pBR322 is substantially undigested because the adenine residues in the recognition site (TCCGGA) are methylated by dam methylase.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning

AccIII (BspMII)

Features

DNA Restriction Enzyme: AccIII (BspMII)


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