IPTG is also commonly used in blue/white screening experiments to determine the presence of absence of bacterial recombinant vectors following transformation. If the insert of interest is cloned into the vector's lacZ gene, it disrupts B-Gal and prevents its expression. Bacterial colonies are grown on media containing X-gal (5-bromo-4-chloro-3-indolyl- beta-D-galactopyranoside), a substrate for B-Gal that turns into an insoluble blue product upon being cleaved by the enzyme. Without B-Gal, bacterial colonies exposed to X-gal cannot process the substrate and remain white, evidence that they are transformed with recombinant vector and not non-recombinant vector. These white bacterial colonies can be selected for subsequent recombinant vector amplification.
Many regulatory elements of the lac operon are used in inducible recombinant protein systems; IPTG is an effective inducer when used in the concentration range of 100 micromolar to 1.5 millimolar. Inducer concentration depends on the required strength of induction as well as the cell or plasmid genotype; for example, if the organism genotype includes lacIq, a mutation that results in the overproduction of the lac repressor, then a higher concentration of IPTG may be necessary.