- Migration buffer for native (non-denaturing) polyacrylamide gel electrophoresis of protein
- Preparation of transfer buffer for Western Blotting (Tris-Glycine/20% methanol)
Tris-Glycine Buffer (TG) Powder, pH8.3 (Cat. # T9102)
10 pouches (for 10 L)
Final solution from one pouch (1 L) contains:
0.025 M Tris
0.192 M Glycine
Tris-Glycine Powder (Cat. # T902)
50 packages (for 25 L)
One package (for 500 mL) contains:
3.0 g Tris (hydroxymethly) aminomethane
14.4 g Glycine
Store powder at room temperature.
Tris-Glycine Powder (Cat. # T902) is manufactured by Roman Industries Co., Ltd.
Dissolve in distilled or deionized water.
1. Davis, B. J. (1964) Ann. N.Y. Acad. Sci. 121