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Products >  Molecular_Biology_Tools >  Reagents >  Agarose >  PrimeGel_Agarose

PrimeGel Agarose for Gel Electrophoresis

PrimeGel Agarose is available in several different formats for creating agarose gels that are optimized for various nucleic acid fragment size ranges and applications, or for electrophoretic analysis of proteins.

The available formats include:

  • PrimeGel Agarose LE 1-20K—high-quality agarose for separating DNA fragments of 1 kb or more. It is suitable for Southern blotting and confirming PCR products.
  • PrimeGel Agarose LE 1-20K GAT—gel purification grade agarose for separating and recovering DNA fragments of 1 kb or more. It is suitable for Southern blotting and confirming PCR products.
  • PrimeGel Agarose GOLD 3-40K—high-quality agarose suitable for pulsed-field gel electrophoresis. It can also be used for immobilization of enzymes and cells, as well as blotting.
  • PrimeGel Agarose LMT 1-20K—low-melting agarose for separating DNA fragments of 1 kb or more. It may be used for recovering fragments to be used in enzymatic reactions such as restriction digestion, ligation and PCR.
  • PrimeGel Agarose LMT 1-20K GAT— gel purification grade, low-melting agarose for separating DNA fragments of 1 kb or more. This agarose may also be used for in-gel enzymatic reactions such as restriction digestion, ligation, and PCR that can be performed directly in the remelted agarose gel without further purification.
  • PrimeGel Agarose PCR-Sieve—high-quality agarose for separating and recovering DNA fragments of 1 kb or less. This agarose is ideal for blotting. It forms sturdy gels that are easy to handle.
  • PrimeGel Agarose PCR-Sieve HRS—high-resolution agarose for separating and recovering DNA fragments of 1 kb or less. This agarose is ideal for blotting and has a resolution comparable to that of a polyacrylamide gel.
  • PrimeGel Agarose LMT PCR-Sieve GAT—gel purification grade, low melting agarose for separating and recovering DNA fragments of 1 kb or less. This agarose can be used for recovering fragments to be used in enzymatic reactions such as restriction digestion, ligation and PCR.
  • PrimeGel Agarose ME PT—high-quality agarose for immuno-electrophoresis and serum protein electrophoresis.
  • PrimeGel Agarose HGT PT— high-quality agarose for preparing agarose beads, as well as immuno-electophoresis and serum protein electrophoresis.

  At-A-Glance   Documents   Images & Data

Features

  • Options for separation of DNA fragments in different size ranges
  • Low melting grade agarose for recovering fragments to be used in enzymatic reactions, or for in-gel reactions
  • Optimized formats to create sturdy gels and provide exceptionally high resolution for blotting
  • Agarose gels and agarose beads designed for the analysis of proteins
Product NameRecommended Gel ConcentrationNucleic Acid Fragment Separation RangeLow Melting Point (LMT)Suitable for Downstream Applications without Additional Purification*
PrimeGel Agarose LE 1-20K0.6–2%200 bp–20 kb  
PrimeGel Agarose LE 1-20K GAT0.6–2%200 bp–20 kb  
PrimeGel Agarose GOLD 3-40K0.3–1.8%500 bp–40 kb  
PrimeGel Agarose LMT 1-20K0.75–1.5%200 bp–20 kbX 
PrimeGel Agarose LMT 1-20K GAT0.75–1.5%200 bp–20 kbXX
PrimeGel Agarose PCR-Sieve2–5%20 bp–1,500 bp  
PrimeGel Agarose PCR-Sieve HRS1.8–5%10 bp–1,200 bp  
PrimeGel Agarose LMT PCR-Sieve GAT2–5%10 bp–1,500 bpXX

* Enables downstream applications, such as direct enzymatic manipulation of nucleic acids, to be carried out in remelted agarose without additional DNA purification. Also enables PCR and sequencing reactions to be carried out in the presence of the remelted agarose gel.

Applications

  • Blotting—PrimeGel Agarose LE 1-20K/1-20K GAT, PrimeGel Agarose GOLD 3-40K, PrimeGel Agarose PCR-Sieve
  • Pulsed-Field Electrophoresis—PrimeGel Agarose GOLD 3-40K
  • High Mobility—PrimeGel Agarose LE 1-20K, PrimeGel Agarose GOLD 3-40K
  • High Resolution—PrimeGel Agarose PCR-Sieve/PCR-Sieve HRS

 
 
Products
Cat. # Product Contents Size Price Units Select
5800A PrimeGel™ Agarose LE 1-20K 100 g $114.00
5801A PrimeGel™ Agarose LE 1-20K GAT 100 g $202.00
5802A PrimeGel™ Agarose GOLD 3-40K 100 g $401.00
5805A PrimeGel™ Agarose LMT 1-20K 25 g $139.00
5806A PrimeGel™ Agarose LMT 1-20K GAT 25 g $155.00
5810A PrimeGel™ Agarose PCR-Sieve 100 g $333.00
5812A PrimeGel™ Agarose PCR-Sieve HRS 100 g $303.00
5815A PrimeGel™ Agarose LMT PCR-Sieve GAT 25 g $117.00
5820A PrimeGel™ Agarose ME PT 100 g $183.00
5821A PrimeGel™ Agarose HGT PT 100 g $557.00
 

 

 
 

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