Binary Vectors for Agrobacterium-mediated Plant Transformation: pRI909 and pRI910
Ri Plasmid pRI909 and pRI910 are binary vectors intended for Agrobacterium-mediated plant transformation and contain a region of T-DNA for Arabidopsis, tomato, tobacco and rice transformation. The origin of the T-DNA is the vir region-lacking Rhizobium (Agrobacterium) rhizogenes Ri plasmid. Ri Plasmid pRI909 and pRI910 are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are capable of high copy number because they contain the ColE1 replication origin. The ColE1 ori, as well as the mutant-type Ri plasmid replication of origin (Ri-ori), are each stably maintained in Rhizobium (Agrobacterium). These vectors also contain the kanamycin resistance gene NPTIII for selection in E. coli and Rhizobium (Agrobacterium) and a mutant-type kanamycin resistance gene NPTII for selection in plants.
The Ri Plasmid pRI909 and pRI910 vectors are capable of plant transformation in combination with Rhizobium (Agrobacterium) through a binary vector system. Stable plant chromosome target gene integration is possible with the Ri Plasmid pRI909 and pRI910 because their cloning sites are located in close proximity to the T-DNA Right Border(RB) and not the NPT II plant selection marker. As a result, the target gene is not deleted.