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Molecular Biology Tools

Products >  Molecular_Biology_Tools >  Nucleic_Acids >  DNA >  M13_mp18_RF_DNA

M13 mp18 RF Phage Vector

The M13 mp18 RF Phage Vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ' region, recombinant vectors can be easily verified via blue/white colony screening using agar plates containing IPTG and X-Gal. The phage vector can also be directly used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles. Our M13 primers (Cat. # 3831, 3832 and 3830) are available for M13 sequencing. Our Deletion Kit for Kilo-Sequencing (Cat. # 6030) can be used  for sequencing of the cloned M13 mp18 RF phage vector DNA.

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  • >70% double-stranded covalently closed circular form I (RF I) DNA.
  • Multiple cloning site integrity verified by dideoxy sequencing analysis.
  • EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III restriction sites verified by restriction enzyme analysis.




Cloning of long DNA fragments (> 4 kb) using the M13 mp18 RF Phage Vector can result in some colony deletions.


10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Product Citations

Messing, J. New M13 vectors for cloning. Methods Enzymol. 101, 20–78 (1983).

Yanisch-Perron, C., Vieira, J. & Messing, J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103–19 (1985).


  • Entry name: M13MP18
  • Accession No.: X02513


0.5 µg/µl

Cat. # Product Contents Size Price Units Select
3118 M13 mp18 RF DNA 10 ug $83.00



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