Takara's Protein Molecular Weight Markers are designed for use with SDS-Polyacrylamide Gel Electrophoresis (PAGE). These protein ladders for SDS-PAGE are available in three size ranges: Low (molecular weight range: 14.3-97.2 kDa), High (molecular weight range: 44.3-200 kDa) and Broad (molecular weight range: 6.5-200 kDa).
Each one of the protein molecular weight markers is quantitated to yield uniform band intensities when stained with Coomassie Brilliant Blue R-250 following SDS-PAGE. Five microliters of 20-fold diluted protein ladder is recommended per each lane of an SDS-PAGE minigel.