- cDNA synthesis (Ribonuclease Inhibitor: 0.5 U/µl reaction)
- In vitro translation (Ribonuclease Inhibitor: 1 U/µl reaction)
- In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 U/µl reaction)
- In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 U/µl reaction)
- Polysome isolation (Ribonuclease Inhibitor: 1,000 U/ml reaction)
2314A: 5,000 U
2314B: 5 x 2314A (25,000 U)
E. coli containing a plasmid that carries the porcine RNase Inhibitor gene
Blackburn, P. Ribonuclease inhibitor from human placenta: rapid purification and assay. J. Biol. Chem. 254, 12484–7 (1979).
Blackburn, P., Wilson, G. & Moore, S. Ribonuclease inhibitor from human placenta. Purification and properties. J. Biol. Chem. 252, 5904–10 (1977).
Burton, L. E. & Fucci, N. P. Ribonuclease inhibitors from the livers of five mammalian species. Int. J. Pept. Protein Res. 19, 372–9 (1982).
Eichler, D. C., Tatar, T. F. & Lasater, L. S. Effect of human placental ribonuclease inhibitor in cell-free ribosomal RNA synthesis. Biochem. Biophys. Res. Commun. 101, 396–403 (1981).
de Martynoff, G., Pays, E. & Vassart, G. Synthesis of a full length DNA complementary to thyroglobulin 33 S messenger RNA. Biochem. Biophys. Res. Commun. 93, 645–53 (1980).
Scheele, G. & Blackburn, P. Role of mammalian RNase inhibitor in cell-free protein synthesis. Proc. Natl. Acad. Sci. U. S. A. 76, 4898–902 (1979).
Turner, P. M., Lerea, K. M. & Kull, F. J. The ribonuclease inhibitors from porcine thyroid and liver are slow, tight-binding inhibitors of bovine pancreatic ribonuclease A. Biochem. Biophys. Res. Commun. 114, 1154–60 (1983).
One unit is defined as the amount of Recombinant Ribonuclease Inhibitor, GPR required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A.
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.