- cDNA synthesis (Ribonuclease Inhibitor: 0.5 units/µL reaction)
- In vitro translation (Ribonuclease Inhibitor: 1 unit/µL reaction)
- In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 units/µL reaction)
- In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 unit/µL reaction)
- Polysome isolation (Ribonuclease Inhibitor: 1000 units/mL reaction)
2313A: 5000 U
2313B: 5 x 2313A (25000 U)
E. coli containing a plasmid that carries the porcine RNase Inhibitor gene
- Burton, L.E. & Fucci, N.P. (1982) Int. J. Pept. Protein Res. 19:372-379.
- Blackburn, P. et al. (1977) J. Biol. Chem. 252:5904-5910.
- Turner, P.M. et al. (1983) Biochem. Biophys. Res. Comm. 114:1154-1160.
- Blackburn, P. (1979) J. Biol. Chem. 254:12484-12487.
- De Martynoff, G. et al. (1980) Biochem. Biophys. Res. Comm. 93:645-653.
- Scheele, G. & Blackburn, P. (1979) Proc. Natl. Acad. Sci. USA 76:4898-4902.
- Eichler, D.C. et al. (1981) Biochem. Biophys. Res. Comm. 101:396-403.
One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3'-cyclic monophosphate (cyclic CMP) by RNAse A.
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.