- Dephosphorylation of DNA 5'-termini
- Reduction of vector re-ligation and background
Pichia pastoris that has been genetically modified with a gene coding ashrimp alkaline phosphatase.
Supplied with 1ml 10X SAP Buffer [500 mM Tris-HCl (pH 9.0), 50 mM MgCl2]
- Rangnar, L. et al. (1991) Comp. Biochem. Physiol. 99B(4):755-761.
One unit is defined as the amount of enzyme required to produce 1 µmol of p-nitrophenol per minute at 37°C (pH9.8) using p-nitrophenylphosphate as the substrate.