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Molecular Biology Tools

Products >  Molecular_Biology_Tools >  Modifying_Enzymes >  Phosphatases >  Alkaline_Phosphatase_Calf_Intestinal

Calf Intestinal Alkaline Phosphatase (CIAP)

Calf Intestinal Alkaline Phosphatase (CIAP) catalyzes the hydrolysis of 5'-phosphate termini from DNA, RNA, dNTPs and rNTPs. CIAP is supplied in a buffer of 10 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2 and 50% glycerol.

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  • Reduction of linear vector self-ligation and vector background during cloning
  • Dephosphorylation of DNA prior to kinase labeling reactions
  • Template preparation prior to 5'-end labeling
  • Protein dephosphorylation






Calf intestinal alkaline phosphatase (CIAP) cannot be completely heat inactivated and therefore it is recommended that the DNA product be purified by gel or spin column purification or phenol-chloroform extraction. 


Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 9.0), 10 mM MgCl2].


  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp 5.72, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

Unit Definition

One unit is defined as the amount of enzyme that generates 1 µmol/min of p-nitrophenol from p-nitrophenylphosphate at 37°C and pH 9.8.


10–30 U/µL

Cat. # Product Contents Size Price Units Select
2250A Alkaline Phosphatase (Calf Intestine) 1,000 Units $80.00
2250B Alkaline Phosphatase (Calf Intestine) 5,000 Units $315.00



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