BAL 31 Nuclease offers two types of nuclease activities. The enzyme acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'-phosphate and 3'-hydroxyl termini. BAL 31 Nuclease also possesses single-stranded DNA and RNA endonuclease activity and is capable of cleaving at DNA/RNA nicks and gaps. The enzyme requires Ca2+ as an essential cofactor for its exonuclease and endonuclease activities.
BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 5 mM MgCl2, 1 mM EDTA and 50% glycerol.