Tetracycline inducible expression using the self-tranducing Tet-Express transactivator.
The Tet-Express System, like Clontech’s Tet-On 3G System, expresses your transgene from a vector containing the tightly-regulated inducible promoter PTRE3G. However, unlike the Tet-On 3G system, you do not need to create a double-stable cell line that expresses the tetracycline transactivator, since the self-transducing Tet-Express transactivator protein is added directly to your cells.