Tetracycline-inducible expression of luciferase using Tet-Express
Basal expression from the PTRE3G promoter is undetectable by Western analysis. HeLa cells containing a stably integrated tetracycline inducible luciferase expression cassette were treated with Tet-Express according to the standard protocol and incubated overnight—in parallel with untreated cells from the same HeLa cell culture. The next day, lysates of treated and untreated cells were prepared and blotted with anti-luciferase and anti-actin antibodies. Basal expression is undetectable by Western analysis, but can be induced to very high levels in the presence of Tet-Express.