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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet_One

Tetracycline Inducible Expression — Tet-One Systems

Before the Tet-One Systems were developed, Clontech’s Tet-On and Tet-Off products all required two separate vectors: one to introduce the doxycycline-responsive transactivator protein into your target cells, and the other to introduce the inducible promoter controlling your gene of interest.

The Tet-One Systems provide both of these components on a single vector. The Tet-On 3G transactivator is expressed in the forward direction from the human phosphoglycerate kinase 1 promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter in the reverse orientation. Compared to the two-vector Tet-On 3G Systems, all previously published all-in-one vectors have shown a low signal-to-noise ratio, typically providing only 50–100-fold induced expression, even in selected clones. Clontech’s Tet-One Systems are based on an all-in-one design that has shown up to 25,000-fold induction (Heinz et al, 2011).

  At-A-Glance   Documents   Images & Data   Resources

Features

  • All-in-one vector design provides all the power of Tet-On 3G in a single vector, making inducible expression as easy as constitutive expression
  • Low background and high maximum induced expression
  • High sensitivity to doxycycline: Near maximum expression at 10 ng/ml doxycycline
  • Inducible expression in a broad range of tissues, because the Tet-On 3G Transactivator is expressed from the human PGK promoter
  • High-titer lentivirus packaging system provided with Lenti-X Tet-One Systems
  • Retro-X System incudes our Retro-X Universal Packaging System

Applications

  • Tightly controlled inducible expression in mammalian cells

References

Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew R., & Schiedlmeier, B. (2011) Retroviral and transposon-based Tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Human Gene Ther. 22(2):166–176.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
631844 Lenti-X™ Tet-One™ Inducible Expression System Each $1,768.00 License Statements *
631847 Lenti-X™ Tet-One™ Inducible Expression System (Puro) Each $1,768.00 License Statements *
634301 Tet-One™ Inducible Expression System Each $1,095.00 License Statements *
634304 Retro-X™ Tet-One™ Inducible Expression System Each $1,598.00 License Statements *
634307 Retro-X™ Tet-One™ Inducible Expression System (Puro) Each $1,598.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Contents Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $390.00
631106 Tet System Approved FBS 500 mL $273.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
631132 TetR Monoclonal Antibody (Clone 9G9) 200 ug $329.00
0027VCT rLV.EF1.Tet3G-9 3 x 20 uL $1,684.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 
 
 

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