TetR Monoclonal Antibody (Clone 9G9) is a monoclonal antibody raised against the full-length wild-type bacterial Tet repressor protein (TetR), which is an essential component of the Tet-On and Tet-Off transactivator proteins.
The TetR antibody can be used for Western blotting and greatly simplifies the process of developing a Tet-inducible expression system by providing a method for early detection of the Tet-On 3G, Tet-On Advanced, and Tet-Off Advanced proteins.
The monoclonal antibody detects the following proteins on a Western blot*:
- Tet-On 3G
- Tet-On Advanced
- Tet-Off Advanced
- Tet Repressor (TetR)
*This TetR antibody is not recommended for use with the original Tet-On or Tet-tTS proteins.
Note: Antibody-based detection of these tetracycline transactivators in cell lines is not a substitute for a functional test. Some highly inducible clones may express tetracycline transactivators at levels that are undetectable on a Western blot.