All lentiviral versions of the Tet-On 3G System contain the following components:
10 µg regulator vector (see tables below)
10 µg response vector (see tables below)
10 µg control vector expressing luciferase under the control of a TRE3G promoter (pLVX-TRE3G-Luc)
Lenti-X HTX Packaging System (Cat. No. 631247)
Lenti-X HTX Packaging Mix 2 (Cat. No. 631260; not sold separately)
Xfect Transfection Reagent (Cat. No. 631317)
Tet System Approved FBS, US-Sourced (Cat. No. 631105)
Lenti-X GoStix (Sample) (Cat. No. 631242; not sold separately)
Store all components except the Lenti-X GoStix at –20°C. Store the Lenti-X GoStix at room temperature.
Use the tables below to pick the specific system that meets your needs. If you prefer a system that uses the human EF1-alpha promoter to express the transactivator protein, please refer to the second table below. EF1-alpha systems are recommended for use in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells, allowing for consistent expression of the tetracycline transactivator in those cells.