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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  TetOn_3G_Lentivirus_Components

Tet-On 3G Inducible Expression Lentiviral Systems—Kit Components

All lentiviral versions of the Tet-On 3G System contain the following components:

  • 10 µg regulator vector (see tables below)
  • 10 µg response vector (see tables below)
  • 10 µg control vector expressing luciferase under the control of a TRE3G promoter (pLVX-TRE3G-Luc)
  • Lenti-X HTX Packaging System (Cat. No. 631247)
    • Lenti-X HTX Packaging Mix 2 (Cat. No. 631260; not sold separately)
    • Xfect Transfection Reagent (Cat. No. 631317)
    • Tet System Approved FBS, US-Sourced (Cat. No. 631105)
    • Lenti-X GoStix (Sample) (Cat. No. 631242; not sold separately)

Store all components except the Lenti-X GoStix at –20°C. Store the Lenti-X GoStix at room temperature.

Use the tables below to pick the specific system that meets your needs. If you prefer a system that uses the human EF1-alpha promoter to express the transactivator protein, please refer to the second table below. EF1-alpha systems are recommended for use in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells, allowing for consistent expression of the tetracycline transactivator in those cells.

Note that plasmid, retroviral, and adenoviral Tet-On 3G inducible expression systems are also available.

Lenti-X Tet-On 3G Inducible Expression Systems with CMV Regulator Vectors
  Core elements common to every vector
  Lentiviral vector backbone for pLVX vectors
Cat. No./Product Regulator Vector Response Vector
631187 pLVX-Tet3G regulator vector pLVX-TRE3G response vector

Lenti-X Tet-On 3G Inducible Expression System

631351 pLVX-TRE3G-IRES bicistronic response vector

Lenti-X Tet-On 3G Inducible Expression System (Bicistronic Version)

631349 pLVX-TRE3G-mCherry IRES bicistronic response vector

Lenti-X Tet-On 3G Inducible Expression System (with mCherry)

631350 pLVX-TRE3G-ZsGreen1 IRES bicistronic response vector

Lenti-X Tet-On 3G Inducible Expression System (with ZsGreen1)

Lenti-X Tet-On 3G Inducible Expression Systems with EF-1 alpha Regulator Vectors
  Core elements common to every vector
  Lentiviral vector backbone for pLVX vectors
Cat. No./Product Regulator Vector Response Vector
631363 pLVX-EF1a-Tet3G regulator vector pLVX-TRE3G response vector

Lenti-X Tet-On 3G Inducible Expression System (EF1alpha Version)

631354 pLVX-TRE3G-IRES bicistronic response vector

Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, Bicistronic)

631352 pLVX-TRE3G-mCherry IRES bicistronic response vector

Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, mCherry)

631353 pLVX-TRE3G-ZsGreen1 IRES bicistronic response vector

Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)

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