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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-tTS_Cell_Lines

Tetracycline-Inducible shRNA- Tet-tTS Cell Lines

Accessory cell lines for Clontech’s Tet-inducible shRNA systems.

Tet-tTS cell lines have been clonally selected to express the Tetracycline transcriptional silencer, or tTS, and greatly simplify the task of creating a tetracycline inducible shRNA cell line, as they are ready-made hosts for our Knockout Tet RNAi Systems H and P.

How tTS Works…

In the absence of doxycycline (Dox), tTS binds tightly to Tet operator sequences (tetO) in the promoter of Clontech’s inducible shRNA vectors. The KRAB domain of tTS then acts as a potent suppressor of shRNA transcription. When Dox is added to the culture medium, tTS dissociates from tetO to allow full promoter activity and high-level shRNA expression.

  At-A-Glance   Documents   Images & Data

Features

  • Cell lines with high inducibility for shRNA
  • Rapidly create an inducible expression system for your shRNA of interest.
  • Premade Tet-responsive cell lines reduce development time/effort in half

Applications

  • Inducible shRNA for gene knockdown in mammalian cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
631146 HEK 293 tTS Cell Line Each $607.00 License Statements *
631147 HeLa tTS Cell Line Each $607.00 License Statements *
631148 MCF7 tTS Cell Line Each $607.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Contents Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $495.00
631106 Tet System Approved FBS 500 mL $465.00
 

 

 
 

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