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Inducible Systems

Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_Cell_Lines

Tetracycline-Inducible Expression-Tet-On Cell Lines

Tet-On cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. Tet-On 3G cell lines, which are our highest performing pre-made cell lines for this system, are located on a separate page.

Tet-Off Advanced and Tet-Off inducible cell lines are also available, which are activated by removing Dox from the culture medium.

These rigorously tested clonal cell lines:

  • Stably express one of three generations of the Tet-On transactivator protein including a limited selection of cell lines that express our premium Tet-On 3G transactivator.
  • Allow you to induce expression of your gene-of-interest by adding doxycycline (Dox) to the culture of transfected cells
  • Can save you up to two months of time in developing a complete Tet-controlled system

Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTRE3G ), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.

  At-A-Glance   Documents   Images & Data   Resources


  • Tet-On 3G cell lines, which are our highest performing pre-made cell lines for this system, are located on a separate page
  • Rapidly create an inducible expression system for your gene of interest
  • Premade Tet-responsive cell lines reduce development time/effort by half


  • Inducible gene expression in mammalian cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price License Units Select
631150 HepG2 Tet-On® Advanced Cell Line Each $678.00 License Statements *
631153 MCF7 Tet-On® Advanced Cell Line Each $678.00 License Statements *
631137 PC12 Tet-On® Cell Line Each $587.00  
631144 T-47D Tet-On® Cell Line Each $587.00  
631143 U2-OS Tet-On® Cell Line Each $587.00  
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.


Required Products
Cat. # Product Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $775.00
631106 Tet System Approved FBS 500 mL $580.00



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