Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Inducible Systems


Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_3G_Retrovirus

Retroviral Tet-On 3G Inducible Expression Systems

This page describes a two-vector retrovirus format for inducible expression. Click this link to learn more about Clontech's all-in-one Retro-X Tet-One Inducible Expression Systems

The Retro-X Tet-On 3G Inducible Expression System combines the efficiency of retroviral gene transfer with the power of our premium tetracycline-regulated gene expression technology. The retroviral vectors in this system can be used to establish a tightly controlled inducible gene expression system in a wide variety of primary cell cultures and immortalized cell lines.

How it Works

Doxycycline-controlled “transactivators” are fusion proteins that contain a DNA-binding TetR domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G, Tet-On Advanced and Tet-Off Advanced proteins). With very high specificity, these transactivators recognize and bind to a Tetracycline Response Element (TRE) that is located in the PTRE3GV promoter upstream your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. Tet-On 3G is the premium 3rd generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.

In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium.

Ideal Retroviral Packaging

Retro-X Tet-On 3G systems are supplied with our Retro-X Universal Packaging System (Cat. No. 631530) which produces high titers of MMLV retrovirus and allows you to customize the tropism of your retrovirus to efficiently transduce any cell line or primary cell culture, and thus greatly expands the applications for these inducible systems.

Retro-X Q Vector Technology

The vectors in these systems feature Clontech’s Retro-X Q-Series technology, which:

  • Drives high titer virus production in packaging cells
  • Inactivates viral LTRs in target cells to prevent promoter interference

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Outstanding induction control and extremely low basal expression for a broad range of dividing cell types
  • TRE3G promoter is optimized for very tight transcriptional control
  • Supplied with our best-performing retroviral packaging system (Retro-X Universal)
  • Tet-Express and Tet-One formats are also available

Applications

  • Establish a tetracycline inducible gene expression system in a wide variety of dividing cells using retrovirus

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
631188 Retro-X™ Tet-On® 3G Inducible Expression System Each $1,598.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions