How it Works
Doxycycline-controlled “transactivators” are fusion proteins that contain a TetR-derived DNA binding domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G). With very high specificity, the transactivator recognizes and binds to a Tetracycline Response Element (TRE) that is located in the PTRE3G promoter of pLVX-TRE3G, into which you clone your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. Tet-On 3G is the premium 3rd generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.
In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium, while in Tet-Off systems, transcription is activated by its withdrawal.
High Titer Lentiviral Packaging
Clontech’s Lenti-X High Titer Packaging System, supplied with each system, generates vastly superior titers than most commercially available packaging systems. You can often transduce an entire dish of target cells with just 10–100 µl of unconcentrated viral supernatant. For more details, visit the Lenti-X HTX Packaging System product page.