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Inducible Systems

Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_3G_Lentivirus

Lentiviral Tet-On 3G Inducible Expression Systems

This page describes a two-vector lentivirus format for inducible expression. Click this link to learn more about Clontech's all-in-one Lenti-X Tet-One Inducible Expression Systems.

The Lenti-X Tet-On 3G Inducible Expression Systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system. The lentiviral Tet-On-3G system consists of two lentiviral vectors: a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the TRE3G promoter that controls the expression of your transgene. Also included are a control response vector for luciferase expression, and our premium Lenti-X Packaging Single Shots (VSV-G).

How it Works

Doxycycline-controlled “transactivators” are fusion proteins that contain a TetR-derived DNA binding domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G). With very high specificity, the transactivator recognizes and binds to a Tetracycline Response Element (TRE) that is located in the PTRE3G promoter of pLVX-TRE3G, into which you clone your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. Tet-On 3G is the premium 3rd generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.

In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium, while in Tet-Off systems, transcription is activated by its withdrawal.

High Titer Lentiviral Packaging

Clontech’s Lenti-X Packaging Single Shots (VSV-G), supplied with each system, generates superior titers than most commercially available packaging systems.

  At-A-Glance   Documents   Images & Data   Resources


  • Low basal expression, high maximal expression, highly controllable
  • Lenti-X Packaging Single Shots (VSV-G), included with each system, generates high titers with a very high safety profile
  • TRE3G promoter provides very tight control of transcription
  • Tet-Express and Tet-One formats are also available


  • Tetracycline-inducible expression in the widest range of hard-to-transfect cell types.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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