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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_3G_IRES_Bicistronic

Bicistronic IRES Tet-On 3G Inducible Expression Systems

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems express two transgenes from our premium TRE3G inducible promoter (PTRE3G). You can either co-express two different genes of interest using systems that contain the pTRE3G-IRES Vector, which contains two multiple cloning sites flanking an IRES sequence (Cat. Nos. 631166 and 631346), or you can monitor co-induced expression of your transgene with a fluorescent protein using kits that contain pTRE3G-mCherry (Cat. Nos. 631165 and 631347) or pTRE3G-ZsGreen1 (Cat. Nos. 631164 and 631348). For details on the exact composition of each kit, please visit the Tet-On 3G kit component page.

These IRES-containing bicistronic vectors allow the simultaneous induced expression of two proteins separately but from the same RNA transcript.

How Does an IRES Work?

The IRES of the encephalomyocarditis virus (ECMV) permits the translation of two open reading frames from one messenger RNA. Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location. Thus, two proteins are expressed simultaneously from the same bicistronic mRNA transcript.
At-A-Glance Documents Images & Data Resources

Features

  • For details on the exact composition of each kit visit the Tet-On 3G kit component page
  • Tightly controlled inducible expression of two genes of interest from the same bicistronic transcript
  • Monitor induced expression of a single gene using mCherry or ZsGreen1 fluorescent proteins
  • Two transgenes or one transgene and a bright fluorescent protein expressed from a single IRES bicistronic transcript
  • You may alternatively be interested in Tet-On 3G systems that contain a bidirectional promoter

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
 
Products
Cat. # Product Package Size Price License # of Units Select
631164 Tet-On® 3G Inducible Expression System (with ZsGreen1) Each Call License Statements *
631165 Tet-On® 3G Inducible Expression System (with mCherry) Each Call License Statements *
631166 Tet-On® 3G Inducible Expression System (Bicistronic Version) Each Call License Statements *
631346 Tet-On® 3G Inducible Expression System (EF1alpha, Bicistronic) Each Call License Statements *
631347 Tet-On® 3G Inducible Expression System (EF1alpha, mCherry) Each Call License Statements *
631348 Tet-On® 3G Inducible Expression System (EF1alpha, ZsGreen1) Each Call License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Package Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $390.00
631106 Tet System Approved FBS 500 mL $273.00
 

 


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