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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_3G_EF1-Alpha_Promoter

EF1-Alpha Promoter Versions of Tet-On 3G

EF-1 alpha promoter versions of the Tet-On 3G Tetracycline Inducible Expression System  provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells. This means that in these cell types long term tetracycline inducible expression can be achieved. The systems contain the same pTRE3G vectors as our standard Tet-On 3G Systems that generate the lowest possible background expression; however the Tet-On 3G transactivator is expressed from an EF1-alpha promoter instead of a CMV promoter. The EF-1 alpha promoter in these systems is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1.

We tested the EF-1 alpha version in Jurkat cells, a cell line known to show reduced expression and clonal variation in expression from CMV-based vectors. When expressing the Tet-On 3G transactivator protein from the EF-1 alpha promoter, 83% of the Jurkat Tet-On 3G clones showed strong inducible expression and 33% demonstrated very high inducibility (greater than 2,000-fold). Such levels of control are not possible when using previous versions of the Tet-On system for this cell line.

At-A-Glance Documents Images & Data Resources

Features

  • View kit components
  • Tetracycline transactivator, Tet-On 3G, is expressed from an EF1-alpha promoter
  • Very low background expression
  • High induced expression

Applications

  • Inducible expression in cell types known to silence CMV promoters, such as hematopoietic cells and stem cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
631167 Tet-On® 3G Inducible Expression System (EF1alpha Version) Each $993.00 License Statements *
631340 Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha Version) Each $993.00 License Statements *
631341 Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, mCherry) Each $1,095.00 License Statements *
631342 Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1) Each $1,095.00 License Statements *
631346 Tet-On® 3G Inducible Expression System (EF1alpha, Bicistronic) Each $993.00 License Statements *
631347 Tet-On® 3G Inducible Expression System (EF1alpha, mCherry) Each $1,095.00 License Statements *
631348 Tet-On® 3G Inducible Expression System (EF1alpha, ZsGreen1) Each $1,095.00 License Statements *
631363 Lenti-X™ Tet-On® 3G Inducible Expression System (EF1alpha Version) Each $1,768.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Package Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $390.00
631106 Tet System Approved FBS 500 mL $273.00
 

 



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