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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_3G

Tet-On 3G Tetracycline-Inducible Expression Systems

The Tet-On 3G Tetracycline-Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

For more details, download our Tet-On 3G technical note.

Lowest Background Ever

Mutations within the PTRE3G promoter have reduced background expression from this tetracycline inducible promoter by 5–20-fold compared to our previous tightest promoter, PTight.

Highest Sensitivity

Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox).

Highest Fold Induction

Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher.

Choice of Tet-On 3G Vector Formats

There are a range of available Tet-On 3G vector formats:

  • The Tet-On 3G Inducible Expression System (EF1α Version), our EF-1 alpha promoter versions of the Tet-On 3G system, provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.
  • Bidirectional promoter versions of the system allow to induce expression of two genes from the same promoter, either two transgenes of your choosing or a single transgene and a fluorescent protein. If your cells turn red or green after adding Dox, this confirms thatyour gene has been turned on and that you have selected ahigh-performing inducible clone.
  • Using IRES technology, we’ve married very tight gene expression control to a bright red fluorescent protein (mCherry) in the Tet-On 3G Inducible Expression System (with mCherry) and a bright green fluorescent protein (ZsGreen1) in the Tet-On 3G Inducible Expression System (with ZsGreen1). 
  • Retroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.

Use Tetracycline-Approved Serum for Optimal Results

With the greatly increased sensitivity of the Tet-On 3G System, it is more important than ever that the serum you use for your studies is guaranteed to be tetracycline-free. Only Clontech performs actual inducibility tests on a sensitive Tet inducible cell line in order to provide an absolute guarantee that your serum will not affect basal expression in your Tet-On 3G experiments. Each Tet-On 3G System is supplied with 50 ml of our premium Tet-Approved FBS.

 
 
Products
Cat. # Product Contents Size Price License Units Select
631337 Tet-On® 3G Bidirectional Inducible Expression System Each $993.00 License Statements *
631339 Tet-On® 3G Bidirectional Inducible Expression System (with ZsGreen1) Each $1,095.00 License Statements *
631338 Tet-On® 3G Bidirectional Inducible Expression System (with mCherry) Each $1,095.00 License Statements *
631168 Tet-On® 3G Inducible Expression System Each $993.00 License Statements *
631166 Tet-On® 3G Inducible Expression System (Bicistronic Version) Each $993.00 License Statements *
631164 Tet-On® 3G Inducible Expression System (with ZsGreen1) Each $1,095.00 License Statements *
631165 Tet-On® 3G Inducible Expression System (with mCherry) Each $1,095.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Contents Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $825.00
631106 Tet System Approved FBS 500 mL $725.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
631180 Adeno-X™ Adenoviral System 3 (Tet-On® 3G Inducible) 10 Rxns $1,868.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 
 
 

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