The Tet-On 3G Tetracycline-Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.
For more details, download our Tet-On 3G technical note.
Lowest Background Ever
Mutations within the PTRE3G promoter have reduced background expression from this tetracycline inducible promoter by 5–20-fold compared to our previous tightest promoter, PTight.
Highest Sensitivity
Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox).
Highest Fold Induction
Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher.
Choice of Tet-On 3G Vector Formats
There are a range of available Tet-On 3G vector formats:
- The Tet-On 3G Inducible Expression System (EF1α Version), our EF-1 alpha promoter version of the Tet-On 3G system, provides for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.
- Using IRES technology, we’ve married very tight gene expression control to a bright red fluorescent protein (mCherry) in the Tet-On 3G Inducible Expression System (with mCherry) and a bright green fluorescent protein (ZsGreen1) in the Tet-On 3G Inducible Expression System (with ZsGreen1). If your cells turn red or green after adding Dox, this confirms that your gene has been turned on and that you have selected a high-performing inducible clone.
- Retroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.
Use Tetracycline Approved Serum for Optimal Results
With the greatly increased sensitivity of the Tet-On 3G System, it is more important than ever that the serum you use for your studies is guaranteed to be tetracycline-free. Only Clontech performs actual inducibility tests on a sensitive Tet inducible cell line in order to provide an absolute guarantee that your serum will not affect basal expression in your Tet-On 3G experiments. Each Tet-On 3G System is supplied with 50 ml of our premium Tet-Approved FBS.
