Tet-Off and Tet-Off Advanced inducible cell lines are host cell lines that greatly simplify the task of setting up tetracycline-inducible gene expression systems which are activated by removing doxycycline (Dox) from the culture medium (1, 2). Our Tet-On Advanced and Tet-On inducible cell lines are also available, which are activated by adding Dox to the culture medium.
These rigorously tested clonal cell lines:
- Stably express either the second-generation Tet-Off Advanced transactivator protein or the original Tet-Off transactivator protein
- Allow you to induce high-level expression of your gene-of-interest by removing (Dox) from the culture of transfected cells
- Can save you up to two months of time in developing a complete Tet-controlled system.
Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.
