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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-Off_Cell_Lines

Tetracycline-Inducible Expression-Tet-Off Cell Lines

Tet-Off and Tet-Off Advanced inducible cell lines are host cell lines that greatly simplify the task of setting up tetracycline-inducible gene expression systems which are activated by removing doxycycline (Dox) from the culture medium (1, 2). Our Tet-On Advanced and Tet-On inducible cell lines are also available, which are activated by adding Dox to the culture medium.

These rigorously tested clonal cell lines:

  • Stably express either the second-generation Tet-Off Advanced transactivator protein or the original Tet-Off transactivator protein
  • Allow you to induce high-level expression of your gene-of-interest by removing (Dox) from the culture of transfected cells
  • Can save you up to two months of time in developing a complete Tet-controlled system.

Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.
  At-A-Glance   Documents   Images & Data

Features

  • Rapidly create an inducible expression system for your gene of interest
  • Premade Tet-responsive cell lines reduce development time/effort in half
  • Cell lines produce excellent inducibility

Applications

  • Inducible gene expression in mammalian cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
631133 CHO AA8 Tet-Off® Cell Line Each $647.00  
631152 HEK 293 Tet-Off® Advanced Cell Line Each $770.00 License Statements *
631141 HT-1080 Tet-Off® Cell Line Each $647.00  
631156 HeLa Tet-Off® Advanced Cell Line Each $770.00 License Statements *
631154 MCF7 Tet-Off® Advanced Cell Line Each $770.00 License Statements *
631134 PC12 Tet-Off® Cell Line Each $647.00  
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Contents Size Price # of Units Select
631101 Tet System Approved FBS, US-Sourced 500 mL $850.00
631106 Tet System Approved FBS 500 mL $840.00
 

 

 
 

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