Tet-Express Inducible Expression Systems are a faster, simpler adaptation of the Tet-On and Tet-Off expression systems. Tet-Express requires only a single vector, the Tet-Express transducible protein, and a doxycycline-free protocol. Tet-Express makes tetracycline-controlled transcription a rapid process for all cell types, and is particularly advantageous for cells that are not amenable to sequential rounds of clonal selection.
Tet-Express Plasmid Systems
All plasmid versions of the Tet-Express System contain the following seven components (store all components at –20°C):
10 µg response plasmid (see table below)
25 rxns Tet-Express (includes the Tet-Express Transactivator and Intensifier Reagent)
10 µg control plasmid expressing luciferase under the control of a TRE3G promoter (either pTRE-Luc or pTRE-BI-Luc)
2 µg Linear Hygromycin Marker (also sold separately as Cat. No. 631625)
2 µg Linear Puromycin Marker (also sold separately as Cat. No. 631626)
100 rxns Xfect transfection reagent (also sold separately as Cat. No. 631317)
50 ml Tet System Approved FBS, US Sourced (also sold separately as Cat. No. 631105)
Tet-Express Lentiviral Systems
All lentiviral versions of the Tet-Express System contain the following components. Store all components except the Lenti-X GoStix at –20°C. Store the Lenti-X GoStix at room temperature.
10 µg response vector (see the second table below)
25 rxns Tet-Express (includes the Tet-Express Transactivator and Intensifier Reagent)
10 µg control vector expressing luciferase under the control of a pLVX-TRE3G promoter (pLVX-TRE3G-Luc)
Lenti-X HTX Packaging System (Cat. No. 631247)
Lenti-X HTX Packaging Mix 2 (Cat. No. 631260; not sold separately)
Xfect Transfection Reagent (Cat. No. 631317)
Tet System Approved FBS, US-Sourced (Cat. No. 631105)
Lenti-X GoStix (Sample)
Use the tables below to pick the specific vector and system that meet your needs. Note that retroviral Tet-Express inducible expression systems are also available.
