Tet-Express Inducible Expression Systems are a faster, simpler adaptation of our powerful, tightly-regulated Tet-On/Tet-Off expression systems. To induce expression, simply apply a few microliters of Tet-Express to the culture medium of cells that have been transduced with pRetroX-TRE3G containing your gene of interest. With very high specificity, the Tet-Express transactivator recognizes and binds to a Tetracycline Response Element (TRE) that is located in the TRE3G promoter of pRetroX-TRE3G and activates transcription.
What is Tet-Express?
Tet-Express is a version of Clontech’s Tet-Off Advanced transactivator protein that has been modified and optimized for self-transduction, i.e., it has the ability to transport itself across cell membranes into the nucleus via protein transduction pathways. Since Tet-Express binds and activates expression in the absence of tetracyclines, doxycycline is not required for gene activation. An Intensifier Reagent included with each vial of Tet-Express increases protein transduction efficiency.
What is Included in the Retroviral Tet-Express Kit?
The Retro-X System is provided with a vial of Tet-Express, the pRetroX-TRE3G Vector to express your gene of interest and a complete retroviral packaging system:
- Tet-Express (includes Tet-Express Transactivator and Intensifier Reagent)
- pRetroX-TRE3G Response Vector
- pRetroX-TRE3G-Luc Control Vector
- Retro-X Universal Packaging System
- GP2-293 Packaging Cell Line
- p10A1 Envelope Vector
- pAmpho Envelope Vector
- pEco Envelope Vector
- pVSV-G Envelope Vector
- pQCLIN Control Retroviral Vector (expresses Beta-Gal)