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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Retroviral_Tet-Express

Tet-Express, Retroviral Format

Tet-Express Inducible Expression Systems are a faster, simpler adaptation of our powerful, tightly-regulated Tet-On/Tet-Off expression systems. To induce expression, simply apply a few microliters of Tet-Express to the culture medium of cells that have been transduced with pRetroX-TRE3G containing your gene of interest. With very high specificity, the Tet-Express transactivator recognizes and binds to a Tetracycline Response Element (TRE) that is located in the TRE3G promoter of pRetroX-TRE3G and activates transcription.

What is Tet-Express?

Tet-Express is a version of Clontech’s Tet-Off Advanced transactivator protein that has been modified and optimized for self-transduction, i.e., it has the ability to transport itself across cell membranes into the nucleus via protein transduction pathways. Since Tet-Express binds and activates expression in the absence of tetracyclines, doxycycline is not required for gene activation. An Intensifier Reagent included with each vial of Tet-Express increases protein transduction efficiency.

What is Included in the Retroviral Tet-Express Kit?

The Retro-X System is provided with a vial of Tet-Express, the pRetroX-TRE3G Vector to express your gene of interest and a complete retroviral packaging system:

  • Tet-Express (includes Tet-Express Transactivator and Intensifier Reagent)
  • pRetroX-TRE3G Response Vector
  • pRetroX-TRE3G-Luc Control Vector
  • Retro-X Universal Packaging System
    • GP2-293 Packaging Cell Line
    • p10A1 Envelope Vector
    • pAmpho Envelope Vector
    • pEco Envelope Vector
    • pVSV-G Envelope Vector
    • pQCLIN Control Retroviral Vector (expresses Beta-Gal)

 
 
Products
Cat. # Product Contents Size Price License Units Select
631177 Tet-Express™ 25 Rxns $229.00 License Statements *
631178 Tet-Express™ 100 Rxns $676.00 License Statements *
631190 Retro-X™ Tet-Express™ Inducible Expression System Each $1,332.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Contents Size Price # of Units Select
631106 Tet System Approved FBS 500 mL $580.00
631306 Puromycin 100 mg $180.00
 

 

 
 

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