Compared to the original Tet-On and Tet-Off Systems, our Tet-Advanced Systems have been optimized for improved expression in mammalian cells by utilizing human codon preferences and removing cryptic splice sites from the mRNA sequence of the transactivator. These enhancements lead to higher and more stable expression levels, while the three minimal VP16 transcription activation domains minimize off-target effects and reduce toxicity. The PTight promoter contains a modified TRE that both reduces basal expression and promotes tight binding to the Tet-Advanced transactivator proteins during induction to permit specific and high-level transcription of your downstream gene
3rd Generation—Tet-On 3G
Tet-On 3G is the most advanced inducible expression system for mammalian cells. The transactivator is similar to Tet-On Advanced but additional mutations leads to further sensitivity to doxycycline. Moreover, sequence optimization within the PTRE3G promoter further reduces basal expression up to 15 fold compared to 2nd generation systems. To read more about Tet-On 3G, please visit the Tet-On 3G product page.