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Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Gen2

Tet-On and Tet-Off 2nd Generation

2nd Generation: Tet-On Advanced and Tet-Off Advanced

Compared to the original Tet-On and Tet-Off Systems, our Tet-Advanced Systems have been optimized for improved expression in mammalian cells by utilizing human codon preferences and removing cryptic splice sites from the mRNA sequence of the transactivator. These enhancements lead to higher and more stable expression levels, while the three minimal VP16 transcription activation domains minimize off-target effects and reduce toxicity. The PTight promoter contains a modified TRE that both reduces basal expression and promotes tight binding to the Tet-Advanced transactivator proteins during induction to permit specific and high-level transcription of your downstream gene

3rd Generation—Tet-On 3G

Tet-On 3G is the most advanced inducible expression system for mammalian cells. The transactivator is similar to Tet-On Advanced but additional mutations leads to further sensitivity to doxycycline. Moreover, sequence optimization within the PTRE3G promoter further reduces basal expression up to 15 fold compared to 2nd generation systems. To read more about Tet-On 3G, please visit the Tet-On 3G product page.

At-A-Glance Documents Images & Data Resources

Features

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631034 pRetroX-Tight-Hyg Vector 20 ug $525.00 License Statements *
631117 pTRE-Cycle3 Vector 20 ug $561.00 License Statements *
632104 Retro-X™ Tet-On® Advanced Inducible Expression System Each $849.00 License Statements *
631115 pTRE-Cycle1 Vector 20 ug $541.00 License Statements *
631114 pTRE-Dual1 Vector 20 ug $541.00  
631113 Tet-Off® Advanced IRES Fluorescent Vector Set 20 ug $598.00 License Statements *
631112 Tet-On® Advanced IRES Fluorescent Vector Set 20 ug $598.00 License Statements *
632163 Lenti-X™ Tet-Off® Advanced Inducible Expression System Each $1,634.00 License Statements *
632162 Lenti-X™ Tet-On® Advanced Inducible Expression System Each $1,634.00 License Statements *
630934 Tet-Off® Advanced Inducible Gene Expression System Each $797.00 License Statements *
630930 Tet-On® Advanced Inducible Gene Expression System Each $797.00 License Statements *
631070 pTet-Off® Advanced Vector 20 ug $536.00 License Statements *
631069 pTet-On® Advanced Vector 20 ug $536.00 License Statements *
631068 pTRE-Tight-BI Vector 20 ug $497.00 License Statements *
631067 pTRE-Tight-BI-ZsGreen1 Vector 20 ug $541.00 License Statements *
631066 pTRE-Tight-BI-AcGFP1 Vector 20 ug $541.00 License Statements *
631065 pTRE-Tight-BI-DsRed-Express Vector 20 ug $541.00 License Statements *
631059 pTRE-Tight Vector 20 ug $446.00   *
632105 Retro-X™ Tet-Off® Advanced Inducible Expression System Each $849.00 License Statements *
631116 pTRE-Cycle2 Vector 20 ug $561.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 

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