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iDimerize Reverse Dimerization System (Inducible Secretion)

The iDimerize Reverse Dimerization System brings the disruption of protein complexes under real-time, small molecule control. A protein of interest is fused to the DmrD binding domain, and the fusion protein molecules aggregate unless the D/D Solubilizer ligand is present. Plasmid and lentiviral (Lenti-X) vector formats are available.

Reverse Dimerization: Disrupting Protein-Protein Interactions

The iDimerize Reverse Dimerization System is a “reverse dimerization” system—aggregation is the resting state, and the D/D Solubilizer breaks up protein-protein interactions. Therefore, the iDimerize Reverse Dimerization System complements inducible dimerization, and can be used in analogous ways to create inducible alleles. In principle, most processes that can be brought under dimerizer control can also be controlled in the reverse manner using this kit to turn off a process that is activated by oligomerization.

Inducible Secretion

The ability to create large protein aggregates has unique applications. For example, adding a secretory signal sequence to fusion proteins allows them to be reversibly stored as aggregates in the endoplasmic reticulum. The ligand can then be added to induce a rapid pulse of protein secretion from the cells. This method has been used to induce rapid, transient and tightly-regulated secretion of human growth hormone (hGH) and insulin (1).

Protein aggregates can also be used in protein trafficking research. For example, this approach has been used to discover the existence of “mega-vesicles” transporting cargo across the Golgi stack (2).

D/D Solubilizer Ligand

The D/D Solubilizer is a synthetic, cell-permeable ligand that can be used to disrupt dimerization of fusion proteins containing the DmrD domain. The D/D Homodimerizer has been tested in vitro and in mice. It is nontoxic. We suggest testing various D/D Homodimerizer concentrations within the recommended range (10–500 nM) for different lengths of time (30 minutes to 12+ hours) in order to obtain a complete dose-response profile. The D/D Solubilizer performs the same function as the AP21998 ligand, which was previously supplied by ARIAD Pharmaceuticals Inc. It is a different molecule than AP21998.

At-A-Glance Documents Images & Data Resources

Features

  • Previously available from ARIAD as the ARGENT Regulated Secretion/Aggregation Kit
  • Brings the disruption of protein complexes under real-time, small molecule control
  • Inducible secretion of proteins
  • Inducible dissociation/disaggregation of proteins
  • Use for protein trafficking studies
  • Plasmid and lentiviral (Lenti-X) vector formats are available

Applications

  • Rapid, reversible changes in the subcellular location, aggregation state and/or biological activity of engineered proteins, in vitro or in vivo
  • Rapid induction of protein secretion
  • Protein trafficking studies
  • Inducible animal models and cell lines


References

  1. Rivera, V. M., et al. (2000) Science 287(5454):826–830.
  2. Volchuk, A., et al. (2000) Cell 102(3):335–348.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
635054 D/D Solubilizer 500 uL $352.00 License Statements
635053 D/D Solubilizer 5 x 500 uL $1,397.00 License Statements
635076 Lenti-X™ iDimerize™ Reverse Dimerization System Each $1,195.00 License Statements
635066 iDimerize™ Reverse Dimerization System Each $1,095.00 License Statements
 

 

 


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