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Inducible Systems

Products >  Inducible_Systems >  Protein-Protein_Interactions >  Heterodimer

iDimerize Inducible Heterodimer System

The iDimerize Inducible Heterodimer System can be used to create and control specific interactions between two different proteins. The proteins of interest are fused to the DmrA and DmrC binding domains respectively, and dimerization is induced by adding the A/C Heterodimerizer (AP21967) to the culture medium or by administering it in vivo. Plasmid and lentiviral (Lenti-X) vector formats are available.

The system can be used to induce an interaction related to any biological process, including but not limited to:

  • Activating heterodimeric cell surface receptors
  • Activating an enzyme by dimerizing two separated domains
  • Directing any protein to a new subcellular location, via an induced interaction with a second protein
    For example, a protein can be targeted to the plasma membrane or nucleus via small molecule-induced interaction with a second protein containing a myristoylation motif or NLS, respectively
  • A major application is the control of transcription—for more information, please visit the regulated transcription page

While it is possible to use the iDimerize Inducible Homodimer System to induce heterodimerization, you will obtain a mixture of homodimers and heterodimers. To induce heterodimers specifically and exclusively, use the iDimerize Inducible Heterodimer System.

A/C Heterodimerizer Ligand (AP21967)

The A/C Heterodimerizer is a synthetic, cell-permeant ligand that can be used to induce heterodimerization of two fusion proteins, one containing the DmrA domain and the other containing the DmrC domain. The A/C Heterodimerizer is identical to AP21967, which was previously supplied by ARIAD Pharmaceuticals, Inc.

iDimerize Inducible Heterodimer System (with Tet-On 3G)

One challenge of ligand-dependent dimerization experiments is that non ligand-induced dimerization events may occur if the proteins of interest are expressed at high levels. This is especially problematic if the target proteins are membrane-bound, because the local concentrations can increase quickly due to the limited space on the membrane. We’ve combined iDimerize and Tet-On 3G technologies to eliminate these unwanted events. First, use doxycycline to optimize the proteins’ expression to physiologically-relevant levels. Then induce dimerization by adding the dimerizer ligand to your culture medium.

  At-A-Glance   Documents   Images & Data   Resources


  • Previously available from ARIAD Pharmaceuticals Inc. as the ARGENT Regulated Heterodimerization Kit
  • Achieve small-molecule control of cellular events that are usually activated by protein heterodimerization
  • Control the localization of a target protein
  • A/C Heterodimerizer is identical to the AP21967 ligand
  • For inducible control of transcription factors, use the iDimerize Regulated Transcription System
  • Plasmid and lentiviral (Lenti-X) vector formats are available


  • Create and control interactions between two different proteins
  • Create conditional alleles of receptors and signaling molecules
  • Activate cell surface receptors or other signaling proteins
  • Recruit signaling proteins to specific subcellular locations (e.g. the plasma membrane or nucleus)
  • Reconstitute enzyme activity by inducing the interaction of separate polypeptides
  • Functional analysis of a signaling pathway in multiple cell types
  • Inducible protein dimerization in animal models and cultured cell lines

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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